Reporter

Part:BBa_K2429032

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


phEF1a mKate

This part contains the sequence for the mKate red fluorescent protein downstream of the human elongation factor 1a (hEF1a) promoter, which is a low level constitutive promoter. For our team's purposes, it will serve as an indicator of how well our RNA binding proteins binds to mRNA by measuring the fluorescence after introducing the protein to the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1814
    Illegal SapI.rc site found at 1196


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