Reporter
Part:BBa_K2429032
Designed by: Nia Myrie Group: iGEM17_MIT (2017-10-24)
phEF1a mKate
This part contains the sequence for the mKate red fluorescent protein downstream of the human elongation factor 1a (hEF1a) promoter, which is a low level constitutive promoter. For our team's purposes, it will serve as an indicator of how well our RNA binding proteins binds to mRNA by measuring the fluorescence after introducing the protein to the cell.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 315
Illegal PstI site found at 820 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 315
Illegal PstI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 569
Illegal XhoI site found at 968 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 315
Illegal PstI site found at 820 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 315
Illegal PstI site found at 820
Illegal NgoMIV site found at 703
Illegal AgeI site found at 81 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1814
Illegal SapI.rc site found at 1196
[edit]
Categories
Parameters
None |